About the Role

We are in search of a Research Scientist II|/Senior Research Scientist with expertise primarily in proteomics workflows for complex matrices (e.g., biosamples) to join our interdisciplinary bioanalytical team here at Sapient. As an RSIII/SRS, you will be responsible for performing laboratory experiments such as the preparation of clinical and biological samples for global/targeted proteomics analyses using medium to high throughput liquid chromatography mass spectrometry instrumentation.

What we’re looking for

For RSIII/SRS:

• Experience with size exclusion chromatography, nanoparticle and other proteomics enrichment preparations, BCA and SPE techniques.
• Familiarity with mass spectrometry techniques (preferably untargeted LC-MS, however targeted LC-MS is also favorable) and working in a BSL2/BSL2+ environment.
• Experience with custom data workflows and data analysis required (i.e., Skyline, Specronaut, mzmine)
• Bachelor's degree in biology, Biochemistry, Chemistry, or a related Life Science field and 3+ years of related work experience or a master’s degree with 1 year of related work experience.

Responsibilities/Skills 

Technical Duties

• Prepares samples using techniques such as size exclusion chromatography, nanoparticle enrichment and solid phase extraction
• Prepares protein samples from a wide range of specimens (e.g., cells, tissue, biofluids, recombinant proteins) for discovery and targeted proteomics analysis
• Conducts global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry, as well as experiments for post-translational modifications (e.g., phosphorylation, acetylation).
• Conducts relative and absolute quantitative analysis using Parallel Reaction Monitoring (PRM) mass spectrometry.
• Uses key software tools for proteomics data analysis (e.g., Spectronaut, Skyline, MSFragger)
• Independently operates laboratory equipment and performs basic troubleshooting and routine maintenance on instruments (e.g., timsTOF Pro 2, Thermo Stellar)
• Assists with developing new methods for testing in the laboratory and maintains detailed records of development, interpretation and results.
• Maintains clear and organized electronic laboratory notebook (ELN), documenting protocols, reagent preparation, and results of all experiments performed in real time.
• Reviews and QCs analytical data using manual and automated pipelines and uses analytical abilities to make decisions regarding the accuracy of results.
• Performs all responsibilities in adherence with regulatory principles and best laboratory practices such as GLP/GCP.

General Duties

• Performs all responsibilities in adherence with regulatory principles and best laboratory practices such as GLP/GCP.
• Establishes excellent working relationships with key collaborators throughout the organization and communicates effectively through appropriate channels and tools.
• Demonstrates the ability to complete projects in a communicative and timely manner under regulatory guidance.
• Maintains safe and efficient procedures in the laboratory.
• Supports the organization, tracking, and storing of specimens.
• Supports management of lab consumables, including ordering, receiving, and storage of consumables.
• Demonstrates the ability to learn complex tasks with instruction and to apply this to perform a variety of new techniques.
• Other duties as assigned

Position Location - San Diego, California (Sorrento Valley)

Position Type - Full-time, On-site

Compensation – $85,000 – 115,000 final compensations will be based on qualification and experience)