Summary:

Vaxcyte’s Research team uses a broad palette of chromatographic tools to purify diverse vaccine antigens. Applicants must have practical, hands-on experience with multiple chromatographic modalities (not limited to monoclonal antibody/Protein A workflows). Experience should include selection, screening, development, scale-up and characterization of various chromatography techniques. 

Essential Functions:

• Execute the following AKTA FPLC chromatography techniques:
  • Immobilized metal affinity chromatography (IMAC)
  • Ion-exchange chromatography (IEX)
  • Hydrophobic interaction chromatography (HIC)
  • Size-exclusion chromatography (SEC)
  • Hydroxyapatite (CHT / HA)
• Designing chromatography trains to reduce host cell protein, endotoxin and aggregates using complementary modalities.
• Demonstrated DoE experience for multimodal optimization is a plus.
• Membrane chromatography for high-throughput contaminant removal or virus/VLP processing and as a scale-friendly polishing/scavenging step.
• Keep accurate and current records of development experiments and/or project related activities in an electronic laboratory notebook.
• Present data from experiments to the team and contribute to technical discussions.
• Work within a team environment and provides support as necessary to further the team’s initiatives.

Requirements: 

• BS in Biochemistry, Molecular Biology or a relevant science considered, with minimum of 2 years of relevant industry experience or MS with minimum of 1 year of relevant industry experience.
• Experience with diverse protein targets, bacterial and eukaryotic recombinant proteins, small/large subunit vaccine antigens, fusion proteins, multimeric complexes, glycoproteins, and VLPs.
• The role requires demonstrated success with alternative capture/polishing methods and orthogonal cleaning strategies appropriate for potential vaccine antigens.
• Preferred experience with Cell-free protein synthesis system.
• Familiarity with automation or high throughput workflows such as (96-well plates) and UNICORN/AKTA software DoE integrations.
• Experience in process development.
• Strong interpersonal skills to collaborate well within the team and excellent written and verbal communication skills.