AbbVie is seeking a driven and innovative Associate Scientist II, in vitro Preclinical Development, to join the mRNA-tLNP group. The Associate Scientist II, in vitro Preclinical Development, will develop and perform cell-based assays to generate data that advance mRNA-tLNP pipeline programs from early discovery through IND-enabling studies

• Execution in vitro assays to evaluate mRNA-tLNP-mediated T-cell reprogramming, including assessment of cytotoxicity, T-cell effector function, and flow cytometry based immune cell phenotyping.
• Isolate PBMCs and T cells from leukopaks.
• Use aseptic techniques to culture mammalian cells, including tumor cell lines, T cells, and PBMCs.
• Make stable tumor cell lines using techniques such as electroporation, viral delivery, and nanoparticle transfections.
• Perform routine tasks competently and independently, and generate reliable and consistent results.
• Demonstrate skill in multiple techniques and appropriately modify protocols, as required, to suit the changing needs of research or development sciences.
• Maintain and implement good laboratory practice including an accurate online laboratory notebook system, instrument and reagent validation, and personal safety precautions.
• Work collaboratively in a cross-functional team environment. Organize and communicate data (written and oral) and participate in project team meetings., with an emphasis on applications in oncology and autoimmune diseases.